The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, raphe nuclei and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/20,000-1/40,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 25 ug of serotonin/BSA. Cross reactivity of Serotonin antisera was examined. With 5µg, 10µg and 25µg amounts the following substances did not react with Serotonin antisera diluted 1/20,000 using the Bn-SA/HRP labeling method: 5-hydroxytryptophan, 5-hydroxyindole -3- acetic acid, and dopamine.Photo Description: Low magnification IHC image of neurons staining for the 5-HT rabbit antibody in the raphe nucleus of the rat brainstem (above), and corresponding high magnification image of the raphe nucleus (below). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:25000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Availability: In Stock

Reacts With: Abalone (Haliotis Kamtschatkana) , Amphibian, Annelid Mushroom, Annelida (Segmented Worm), Ant, Ant (Pheidole Dentata), Antalis Entalis Scaphopoda, Aplysia (Sea Slug), Aplysia Californica (Sea Slug), Aplysia Punctata (Sea Slug), Arachnocampa (Gnat), Archidoris Pseudoargus (Sea Slug), Asterina Pectinifera (Starfish ), Astyanax Mexicanus (Cave Fish), Axolotl (Salamander), Bacteria, Beetle, Bird, Brachiopods, Bryozoans (Polyzoa), Buffalo, Bufo Marinus (Toad), Bug, Bumblebee, Butterfly, Butterfly (Monarch), Canine, Cat, Chick, Chicken, Chiton, Chrysoperla Carnea Green Lacewing , Clam, Clione Limacina Pteropod (Fruit Bat), Cockroach, Cod, Crab, Crayfish, Cricket, Crustaceans, Cupiennius Salei (Spider), Desert Locust, Dog, Dogfish (Shark), Dove, Drosophila (Fly), Drosophila Melanogaster (Fly), Feather Star, Ferret, Fish, Fish (Salmon), Flounder, Fly, Frog, Frog (Xenopus Laevis), Fruit Fly, Gastropod (Slug), Gastropod (Slug), Gerbil, Glowworm, Goldfish, Grasshopper, Guinea Pig, Hamster, Hedgehog, Helisoma Trivolvis (Snail), Hen, Hermit Crab, Honeybee, Human, Idiosepius Notoides (Squid), Ilyanassa Obsoleta (Sea Snail), Insect, Kitten (Cat), Lamprey, Leech, Lizard, Lobster, Lobster (Cycliophora), Lobster Larva, Locust, Lythrypnus Dalli (Fish), Macracantha (Cephalocarida), Manduca Sexta (Moth), Marmoset (Monkey), Mercenaria (Clam), Mexican salamander (Ambystoma  Mexicanum) , Mollusca, Mollusk, Monkey, Monkey (Macaque), Mosquito, Mosquito (Aedes Aegypti), Moth, Moth (Manduca Sexta), Mouse, Mudpuppy, Mussel, Newt, Nudibanch, Nudibranch Mollusc, Nudipleura, Octopus, Onychophora (Velvet Worm), Ovine (Sheep), Parhedyle Cryptophthalma (Sea Slug), Phoronis Pallida (Phoronida), Phyllodoce Maculata (Phyllodocidae), Phyllodoce Maculata (Polychaeta), Pig, Pigeon, Pilidium Larvae, Platynereis Dumerilii (Ragworm), Platypus, Pleurobranchaea Californica (Sea Slug), Pleurobranchaea Japonica (Sea Slug), Pleurodeles (Newt), Prawn, Praying Mantis, Ptychodera Flava (Worm), Quail, Rabbit, Raja Erinacea (Skate), Ram (Sheep), Rana Perezi (Frog), Rat, Red Abalone, Red Abalone, Renilla Koellikeri (Cnidarian ), Rhodnius Prolixus (Bug), Rhodnius Prolixus (Parasite), Salamander, Sand Dollar, Schistocerca Gregaria (Locust), Scorpaena Porcus (Fish), Scorpion, Scyliorhinus Canicula (Dogshark), Sea Bass (Fish), Schistocerca Gregaria (Locust), Scorpaena Porcus (Fish), Scorpion, Scyliorhinus Canicula (Dogshark), Sea Bass (Fish), Sea Cucumber, Sea Lamprey, Sea Lily, Sea Scallop, Sea Slug, Sea Spider, Sea Star, Sea Urchin, Seahorse, Shark, Sheep, Shrew, Skate, Snail, Sparus Aurata (Fish), Spisula (Clam), Springtails, Squirrel Monkey, Sting Ray, Stomatopod Crustacean, Sturgon, Tapeworm, Tectura Scutum (Mollusk), Tick, Tigriopus Californicus (Copepod), Toad, Tragulus Javanicus (Mouse Deer), Tritonia Diomedea (Sea Slug), Trout, Turkey, Turtle, Worm, Worm (Digenea) , Worm (Diphyllobothrium Dentriticum) , Worm (Dugesia Tigrina) , Worm (Dugesia Tigrina), Worm (Phyllodoce Groenlandica), Worm (Polychaete Capitella), Zebra Finch (Bird), ZebrafishAlternate Names:  anti-5-HT RabbitDRRI:  AB_572263Immunogen: SerotoninGene Symbol: SplEntrez Gene ID: 104153

阿尔茨海默氏症协会称，阿尔茨海默氏症的研究抗体在帮助科学家找到解决这种疾病和其他痴呆症的方法方面发挥着重要作用，这些疾病和痴呆症影响了全世界4700万人和530万美国人，到2050年，这一数字有望增加两倍。随着婴儿潮一代的年龄增长，预计将有2800万人患上这种疾病，其费用将升至美国医疗保险总支出的近四分之一。

自从Aloysius Alzheimer博士于1906年首次描述这种疾病以来，科学家们在了解这种疾病如何影响大脑方面取得了显着进步。十多年后，理解和治疗的突破为阿尔茨海默氏病患者带来了希望。

这些ImmunoStar抗体已在阿尔茨海默氏症的研究中得到证明。用关键字“阿尔茨海默氏病” 搜索我们出版物的数据库，以查找数百种引用这些抗体的出版物。有关神经科学家的意见，请参阅我们的客户推荐书，以赞扬他们从ImmunoStar获得的结果和服务。我们的抗体稳定，高滴度，并且批次间一致，可确保成功染色并获得极佳的价值。