The ImmunoStar serotonin (5HT) transporter was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat raphe nuclei, hypothalamus, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/10,000 - 1/15,000 in PBS/0.3% Triton X-100- Biotin/avidin-HRP.By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band. The predicted molecular weight of serotonintransporter based on its nucleic acid sequence is 67,815. The actual weight that one might observefrom tissue extracts could be higher due to glycosylation. We validated the antibody with rat SERT overexpressed in an insect cell expression system. The MW from that was about 67 kD. Our SERT antibody dilution was 1:20,000 using chemiluminescent detection.

Immunolabeling is completely abolished by pre-adsorption with synthetic rat 5HT transporter (602-622).

Photo Description: IHC image of neurons staining for the 5-HT transporter in the raphe nucleus of the rat brainstem (above). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Florescent secondary (below)

Host:Rabbit

Quantity / Volume: 100 µL

State:Lyophilized Whole Serum

Availability: In Stock

Reacts With: Human, Marine, Monkey, Mouse, Rat, Sparrow

Alternate Names: 5HTT; HTT; Sodium-dependent serotonin transporter; Solute carrier family 6 member 4; Slc6a4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4

RRID: AB_572209Immunogen: Rat 5-HT transporter (579-599)Gene Symbol: Slc6a4Database Links:Entrez Gene: 6532 HumanEntrez Gene: 15567 MouseEntrez Gene: 25553 RatEntrez Gene: 102068406 Sparrow

阿尔茨海默氏症协会称，阿尔茨海默氏症的研究抗体在帮助科学家找到解决这种疾病和其他痴呆症的方法方面发挥着重要作用，这些疾病和痴呆症影响了全世界4700万人和530万美国人，到2050年，这一数字有望增加两倍。随着婴儿潮一代的年龄增长，预计将有2800万人患上这种疾病，其费用将升至美国医疗保险总支出的近四分之一。

自从Aloysius Alzheimer博士于1906年首次描述这种疾病以来，科学家们在了解这种疾病如何影响大脑方面取得了显着进步。十多年后，理解和治疗的突破为阿尔茨海默氏病患者带来了希望。

这些ImmunoStar抗体已在阿尔茨海默氏症的研究中得到证明。用关键字“阿尔茨海默氏病” 搜索我们出版物的数据库，以查找数百种引用这些抗体的出版物。有关神经科学家的意见，请参阅我们的客户推荐书，以赞扬他们从ImmunoStar获得的结果和服务。我们的抗体稳定，高滴度，并且批次间一致，可确保成功染色并获得极佳的价值。